CEME-0200 CEME System - Portable Medium Replacement System - AC Adapter - 125ml Bottles
Automates culture medium replacement at low cost!
Suitable for applications where constant and frequent medium replacement is required. (e.g. iPS cell culture)
AC Adapter
2 pcs. 125ml Medium Bottles
about 3-4 weeks
Only %1 left
Article no
CEME-0200
$ 1,784.00
- Automatic medium replacement: The culture medium is replaced automatically at a specified interval (3, 6, 12, or 24 hours).
- Can be used in an incubator: The system can operate continuously for up to 7 days with batteries, and can be placed in an incubator without any wiring.
- Accommodates a standard 6-well plate: A standard 6-well plate can be used, which reduces running costs and minimizes environmental changes resulting from introducing the system.
- Observable on a microscope: The 6-well plate can remain in the system and be placed on a microscope stage when you observe the cells, which also reduces environmental changes.
- Disposable: All system components that are exposed to the medium are replaceable.
| Outer Dimensions | W194 × D228 × H140 mm |
|---|---|
| Other Wetted Materials | Silicone, SUS304, or PP |
| Weight | Approximately 500 g (*Medium and AC adaptor weight are not included) |
| Power Supply | External power supply (AC adaptor) / AA Alkaline Battery |
| Applicable Culture Vessel | 6 Well Plate*1 |
| Initially Required Medium Volume (Per Well) | 3.0 mL*2 |
| Volume Replaced (Per Well) | 2.7 mL*2 |
| Medium Replacement Interval | 12h / 24h / 48h / 72h |
| Notes |
The volume of the medium bottle is 125 mL or 15 mL centrifuge tube..
Note: Details such as specifications, etc., may be changed without notice. *1: Recommended Well Plate: Thermo Fisher Scientific Inc.:NUNC #140675, Sumitomo Bakelite Co., Ltd. MS-80060 *2: Volume may slightly vary depending on the type of well plate. This product is customizable. |
APPLICATION EXAMPLES
Case 1. hiPSC (Human-Induced Pluripotent Stem Cells)
Colony growth was successfully observed for 4 days after passage (engraftment), during which the culture medium was automatically replaced by our system.
[EXPERIMENT CONDITION]
- Cells: 253G1 Human iPS cells (NHDF reprogrammed by 3 factors; Oct3/4, Sox2, Klf4)
- Feeder Cells: MMC treated SNL76/7 cells
- Medium: DMEM-12 (including KSR, NEAA, PSG, 2-ME, bFGF)
Note: Data courtesy of Kidoaki S. Lab, Institute for Materials Chemistry and Engineering, Kyushu University (Japan).
Case 2. Embryoid Bodies (EB) of mouse iPS cells
iPS cells were cultured in Knockout D-MEM-based myocardial differentiation medium using our system, and pulsation of differentiation-inducted cardiomyocytes derived from mouse iPS cells was observed.
Note: Data courtesy of Fujii T. Lab, Institute of Industrial Science, The University of Tokyo (Japan).
Case 3. BrainXell
BrainXell’s summary on comparative experiment between Takasago’s Portable Medium Replacement System and traditional medium replacement methods in induced pluripotent stem cells (iPSCs) maintenance.
Note: Data courtesy of Ms. Sondra Minter, BrainXell, MADISON, WI.
Note: Details such as specifications, etc., may be changed without notice.